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. 2010 Apr 26;5(4):e10351. doi: 10.1371/journal.pone.0010351

Figure 2. Expression of Gprc5b_v2 is enriched in brain.

Figure 2

A. Total mRNA from C57Bl/6J mouse tissues (a 2-month old male) was amplified with Gprc5b primer sets C/c (upper panel) and B/f (middle panel) to detect Gprc5b_v1 and _v2. Primer set B/f can theoretically detect both _v1 and _v2. However, the Gprc5b_v1 product was rarely detected, due to its large size (∼2.7 kb). B. Total mRNA from human tissues (a 72 year old male) was amplified with Gprc5b primer set A/e to detect Gprc5b_v2. See Fig. 1A for relative positions of primers on the transcripts. Gapdh mRNA was amplified as control (bottom panels in A and B).