Figure 3. Mouse strain-specific expressions of Gprc5b splice variants.

Total mRNA from C57Bl/6J, 129/SvJ, and 129/SvImJ forebrains was reverse transcribed, and then amplified with Gprc5b primer sets B/d/f (top panel), and C/c (middle panel) to detect Gprc5b_v1 and/or _v2. See Fig. 1A for relative positions of primers on the transcripts. Gapdh mRNA was amplified as control (bottom panel). No RT: mRNA that was not reverse transcribed into cDNA. Mice were approximately 2 months old when tissues were harvested for RNA isolation.