Skip to main content
. 2010 Apr 26;5(4):e10317. doi: 10.1371/journal.pone.0010317

Figure 5. Peptides with BCAAs increase the recombinant protein production in GCDM grown cells.

Figure 5

(A) Effect of supplementation of GCDM with dipeptides on the expression levels of the ABC transporter Jhp0757 and the SBDs of the ABC transporter GlnPQ (SBD1-2 of GlnP) in L. lactis NZ9000 cells. Cells were induced at OD600≈0.5 with 0.1% volume of nisin A-containing NZ9700 culture supernatant and growth was continued for 2 h. Protein levels were analyzed on immunoblots, using an anti-His tag antibody. Growth media used are indicated above the lanes. (B, C and D) Effect of peptides on the expression level of the soluble protein OpuAC-GFP in L. lactis NZ9000 grown in GM17, GCDM and GCDMPDC or GCDM supplemented with peptides. GCDM supplemented with di-/tripepides containing at least one branched-chain amino acid are indicated by white bars. Whole cell GFP fluorescence of L. lactis NZ9000 carrying pNZ(OpuAC-GFP) was used to quantitate OpuAC-GFP expression levels. Amino acids are indicated by their one-letter code.