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. 2010 Apr 26;5(4):e10317. doi: 10.1371/journal.pone.0010317

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Marreddy et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Overexpression of recombinant proteins in L. lactis NZ9000 and NZ9000Δs cells grown in GM17 and GCDM. Cells were induced at OD₆₀₀≈0.5 with 0.1% volume of nisin A-containing NZ9700 culture supernatant and growth was continued for 2 h. Protein levels were analyzed on immunoblots, using an anti-His tag antibody.