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. 2010 Apr 26;5(4):e10317. doi: 10.1371/journal.pone.0010317

Table 1. Summary of the plasmids used in this study.

Plasmid Gene Function of protein Organism Reference
pNZ(opuAHis) opuA Transport of glycine betaine Lactococcus lactis [37]
pNZ(opuACHis) opuAC SBD of OpuA Lactococcus lactis [38]
pNZ(opuAC-GFPHis) opuAC-GFP SBD of OpuA fused to GFP Lactococcus lactis This work
pNZ(ribUHis) ribU Transport of riboflavin Lactococcus lactis [39]
pNZ(ribCHis) ribC Conversion of riboflavin to cofactor (FMN and FAD) Lactococcus lactis Laboratory stock
pNZ(SBD1&2HisglnP) glnP(SBD1&2) SBDs of glutamine transporter GlnPQ Lactococcus lactis Laboratory stock
pREcLIC(jhp0757His) jhp0757 Putative osmoregulatory transporter Helicobacter pylori This work
pREcLIC(proWXHis) proWX Transport of choline Streptococcus pneumoniae This work
pREcLIC(arpJHis) arpJ Function unknown Listeria monocytogenes This work
pREcLIC(glnPHis) glnP Transport of glutamine Lactococcus lactis This work
pREcLIC(sav1866His) sav1866 Multidrug transporter Staphylococcus aureus Laboratory stock
pREcLIC(bcaP-gfpHis) bcaP-gfp Transport of branched chain amino acids Lactococcus lactis Laboratory stock
pIL252(P32-bcaP-gfp) & pIL253(P32-bcaP-gfp) bcaP-gfp Transport of branched chain amino acids Lactococcus lactis This work
pIL252(P32-bcaP-gfpHis) & pIL253(P32-bcaP-gfpHis) bcaP-gfp Transport of branched chain amino acids Lactococcus lactis This work

*NBD, Nucleotide-binding domain; TMD, Transmembrane domain; SBD, Substrate-binding domain.

pNZ-derived plasmids are based on pNZ8048 [18], pREcLIC-derived plasmids are based on [2] and pIL-derived plasmids are based on [30]. His, indicates the hexa histidine tag engineered at the C-terminal of the protein.