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. Author manuscript; available in PMC: 2010 Apr 26.
Published in final edited form as: Neuroimmunomodulation. 2008 Aug 5;15(2):117–124. doi: 10.1159/000148194

Figure 3.

Figure 3

Relative expression of IL-6 transcripts in C6 astrocytoma cells treated with GABA, TNF-α and IL-1β. Rat C6 cells (3 × 106 cells/dish in 35 × 10 mm dishes) were pre-treated with 1 mM GABA for 1 h and co-treated with either 1 mM GABA, 50 ng/mL IL-1β and 100 ng/mL TNF-α for 4 h. Panel A. Post stimulation, cellular RNA was extracted and 1 μg of total RNA was subsequently separated and analyzed via semi-quantitative RT-PCR. Panel B. IL-6 transcript relative expression levels were determined. TI significantly stimulated IL-6 transcription compared to control in each experiment (P ≤ 0.001). The data are presented as the observations obtained from four experiments.