Table I.
Comparison of the EAH kinetic constants for its initial substrate (EA) and two possible monohydroxylated intermediates
Substrate | Product | Km | kcat | kcat/Km |
---|---|---|---|---|
μM | s−1 | s−1/μM | ||
EA | CD | 19.18 ± 2.90 | 0.493 ± 0.039 | 0.0257 |
3α(OH)EA | CD | 6.38 ± 0.42 | 0.200 ± 0.004 | 0.0313 |
1β(OH)EA | CD | 1.74 ± 0.08 | 0.582 ± 0.006 | 0.3344 |