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. Author manuscript; available in PMC: 2010 Apr 26.
Published in final edited form as: J Biol Chem. 2004 Nov 2;280(5):3686–3696. doi: 10.1074/jbc.M411870200

Table I.

Comparison of the EAH kinetic constants for its initial substrate (EA) and two possible monohydroxylated intermediates

Substrate Product Km kcat kcat/Km
μM s1 s1/μM
EA CD 19.18 ± 2.90 0.493 ± 0.039 0.0257
3α(OH)EA CD 6.38 ± 0.42 0.200 ± 0.004 0.0313
1β(OH)EA CD 1.74 ± 0.08 0.582 ± 0.006 0.3344

Constants were calculated by nonlinear regression fits to the Michaelis-Menten equation using the data from Figs. 1 and 2. CD, capsidiol reaction product.