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. Author manuscript; available in PMC: 2010 Apr 26.
Published in final edited form as: N Engl J Med. 2008 Feb 14;358(7):709–715. doi: 10.1056/NEJMoa073443

Figure 2. Kisspeptin-Stimulated Production of Inositol Phosphate in COS-7 Cells Transfected with Wild-Type GPR54 or Arg386Pro GPR54.

Figure 2

Total inositol phosphate accumulation was measured in counts per minute (cpm) 45 minutes after stimulation with 10−11 to 10−7 M of kisspeptin-10 (Panel A). In a separate experiment, cells were stimulated with 10−9 M of kisspeptin-10 for 0, 2, 4, or 18 hours; the resulting inositol phosphate levels are expressed as the percentage of the maximal level (at 2 hours) (Panel B). Each point is the mean of five independent experiments, each performed in duplicate or triplicate. Finally, cells were transfected with 50 ng of wild-type GPR54 only, 50 ng of Arg386Pro GPR54 only, or 25 ng of wild-type GPR54 and 25 ng of Arg386Pro GPR54. The total inositol phosphate accumulation was measured after stimulation with 10−8 M of kisspeptin-10 for 0, 1, 2, or 4 hours (Panel C). I bars represent the standard errors.