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(A). Schematics of PCR amplification of gals1 DNA sequence with Tev cleavage site, 6HisTag, and multicloning site (MCS). (B). Schema of pLgals1-Tev vector construct. (C). Schema of pLgals1-hum-βGal-T7 construct, the pLgals1-Tev sequence fused to human β4Gal-T7 sequence.
