Table 1.

Comparison of the yields of soluble and folded β4Gal-T7 protein per 1 liter of bacterial culture using MBP and galectin-1 as fusion partners. The yield of the fusion proteins from 1 liter of bacterial culture is 40 mg for MBP-hum-β4Gal-T7 and 15 mg for galectin1-hum-β4Gal-T7. MBP-hum-β4Gal-T7, in contrast to galectin-1-hum-β4Gal-T7, precipitates out of solution before and after protease cleavage and cannot be stored for longer periods of time.

	Alpha-lactose	UDP-agarose column
hum-Galectin-1-hum-β4Gal-T7	15 mg	7.5 mg
	Alpha-amylose	UDP-agarose column
MBP-hum-β4Gal-T7	40 mg	5 mg