Figure 6. Ndi1 expressing flies are resistant to the endogenous complex I inhibitor rotenone and the free radical generator paraquat.
A-C, UAS-Ndi1/Elav-GeneSwitch flies were fed with 2 μg/ml RU486 during development and 10 μg/ml RU486 during the adulthood stage; uninduced flies were fed with diluent. Survival curves are shown as induced (+RU486, solid circles) or uninduced (-RU486, open circles). All assays were performed on female flies between 6-10 days of age. A Ndi1 confers tolerance to 250 μM rotenone (p< 0.0001) and B 30 mM paraquat (p< 0.0001), but does not affect C starvation resistance (p=0.9278). The significance of the difference between survival curves was analyzed using the log-rank statistical test.