Skip to main content
. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Gastroenterology. 2010 Jan 25;138(5):1943–1953. doi: 10.1053/j.gastro.2010.01.032

Figure 4.

Figure 4

Role of HuR, methyl-HuR and AUF1 during liver development. A, B) mRNA expression of MAT2A, HuR, MAT1A and AUF1 in fetal livers (E16, E18), and livers from pups (P1 and P5) and adult rats (3 months), normalized to GAPDH mRNA, p<0.05, *ages of development versus E16. C) Levels of HuR, methyl-HuR and AUF1 as evaluated by Western blot analysis. Ponceau S staining was used as loading control (Supplemental Figure 3). The ratio of methyl-HuR/HuR was calculated, *p<0.05, ages of development versus E16. D, E) Binding of HuR or AUF1 to target mRNAs during liver development, as assessed by RNP-IP and real-time PCR analysis, *p<0.05, ages of development versus E16. F) RNP-IP analysis of MAT2A mRNA bound to methyl-HuR, enrichment represents the average from triplicate experiments, *p<0.05, ages of development versus E16.