Table 4.

SPR sandwich assay of capsid proteins^a

Ligand (Mab)	Analyte-1 (Antigen)	Analyte-2 (Mab)	Binding	Commentb
904	Cp149d	e6	+
		904	-	Binding to single epitope
		905	+
		3105	+
905	Cp149d	e6	+
		904	+
		905	-	Binding to single epitope
		3105	+
		3120	-	No binding to dimers
	Cp149c	e6	-	No binding to capsids
		904	+
		905	+	Multiple epitopes available
		3120	+
e6	Cp149d	e6	+	Binding to two epitopes
		904	+
		905	+
		3105	+
		F11A4 (Fab)	+
		4B5c	-	Negative control

^aLigand (Mab) is immobilized on Biacore chip; capsid protein (Analyte 1) is captured by ligand and used to monitor binding of second Mab (Analyte 2). See Fig. 3 for typical sensogram tracing.

^bComments refer to binding of Analyte-2.

^cThe Mab 4B5 is anti-HBsAg.