Table 1.
TCR γδ expressing hybridomas responsive to insulin peptide B:9–23
| Mouse strain; tissue | Immunization | Hybridoma | TCR-Vγ/Vδ | B:9–23 Response& |
|
|---|---|---|---|---|---|
| NOD.BDC12-4.1 TCR-αtg/Cα−/−; spleen |
Yes | SP9D11 | TRGV4/TRAV4-2 | Vγ4/ Vδ10-like | + |
| NOD.BDC12-4.4 TCR-αtg/Cα−/− retrogenic NOD.scid; pancreatic lymph node |
No | Pln1B5.2 | TRGV1/TRAV15D1DV6D-1 | Vγ1/ Vδ6.3 | + |
| Pln2B8.5 | TRGV1/TRAV6-7/DV9 | Vγ1/ Vδ2.3(Vα4) | + | ||
| Pln5B8.2 | TRGV1/TRAV15D-1DV6D1 | Vγ1/ Vδ6.3 | + | ||
| Pln1B11.13 | TRGV2/TRDV5 | Vγ2/ Vδ5 | + | ||
| Pln3C5.3 | TRGV4/? | Vγ4/ TCR-δ+, Vδ? | + | ||
| NOD; pancreatic lymph node |
No | Pln1B8.1 | TRGV1/? | Vγ1/ TCR-δ+, Vδ? | + |
| Pln1B11.2 | TRGV1/TRAV15D-2/DV6D-2*05 | Vγ1/ Vδ6(λ12-like) | + | ||
| Pln2C11.5 | TRGV1/TRV2-2 | Vγ1/ Vδ4 | + | ||
| C57BL/10; liver | No | 96BLT-8 | TRGV4/TRAV13-4/DV7 | Vγ4/ Vδ7 | − |
| 96BLT-15 | TRGV4/TRDV5 | Vγ4/ Vδ5 | − | ||
| 96BLT-14 | TRGV1/TRAV13-4/DV7 | Vγ1/ Vδ7 | − | ||
| 96BLT-13 | TRGV4/TRDV2-2 | Vγ4/ Vδ4 | − | ||
Immunization: B:9–23/IFA i.p., 2×
Nomenclature: according to IMGT (* designates alleles) and Heilig & Tonegawa (see Methods).
&
All hybridomas responded to TCR cross-linking with anti CD3ɛ mAbs. Responses were measured using a LacZ activity assay and IL-2 ELISA.