FIG. 5.

Coelectroporation of GFP and mCherry expression plasmids. (A, B) Mixing together equal concentrations of two different expression plasmids—one for GFP and one for mCherry—leads to coexpression of GFP and mCherry in large regions of the early developing brain. Combined bright-field and fluorescent images show overlapping expression of GFP (A) and mCherry (B) 24 h after electroporation. Scale bars equal 100 μm. (C) Quantification of the degree of coexpression. Single cells were examined for GFP and mCherry expression. Colored bars represent the percentage of cells coexpressing both GFP and mCherry in a single embryo (20–50 cells/embryo). Bars are color-coded to investigator (blue: S.A.; red: S.K.). Gray bar represents the mean number of cells coexpressing both GFP and mCherry (97.3%; n = 16 embryos; standard deviation = 4.5%).