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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Microb Pathog. 2010 Mar 19;48(6):230–238. doi: 10.1016/j.micpath.2010.03.006

Figure 3. Transmission electron microscopy (TEM) of ETEC strains expressing longus.

Figure 3

Panel A. Wild-type E9034A ETEC strain grown under optimal conditions for longus expression showing typical longus filaments. Panel B. Isogenic E9034AΔlngA mutant strain lacking pili. Panel C. E9034AΔlngA (pAClngA) complemented mutant strain showing Longus-like structures. Panel D. Immunogold labeling TEM of E9034A ETEC wild-type strain. Panel E. Immunogold labeling of E9034AΔlngA mutant strain. Panel F. Immunogold labeling of E9034AΔlngA (pAClngA) complemented mutant strain. Arrows indicate gold particles specifically attached to type IV pili. Scale bar size indicated in μM.