Figure 5. Effect of longus expression on ETEC survival after exposure to antimicrobials.
Panel A. Effect of antimicrobials on bacterial suspensions preincubated in LB. After overnight incubations all suspensions were processed to determine CFU/ml. Panel B. Effect of antimicrobials on bacterial suspensions preincubated in TB. Antimicrobial concentrations used: Chloramphenicol (Cm) at 24 μg/ml, Gentamicin 96 μg/ml, Tetracycline 24 μg/ml, Kanamycin 192 μg/ml, and lysozyme 768 μg/ml. Black bars: E9034A: wild type ETEC strain; white bars: E9034AΔlngA mutant; and striped bars: E9034A:lngA(pBADlngA) complemented mutant. Error bars correspond to standard deviations from experiments done in triplicate.