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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Gastroenterology. 2010 Jan 25;138(5):1920–1930. doi: 10.1053/j.gastro.2010.01.007

Figure 2. RB-independent cell cycle arrest of hepatoma cells by CDK4/6 inhibition.

Figure 2

(A) HepG2 and Huh7 cells models were treated with 1µM PD-0332991 for 24 hours. BrdU incorporation was analyzed by bivariate flow cytometry, data are from at least three independent experiments; bars, SD. (B) Huh7 cells were infected with Ad-p16ink4a or Ad-GFP for 24 hours. BrdU incorporation was analyzed as in (A). (C) Hep3B cells were treated and analyzed as in (A). (D) Following treatment, cell lysates were analyzed by immunoblot for the indicated proteins. (E) Cells were treated as in (A). Cell lysates were analyzed by immunoblot for the indicated proteins. (F) Hep3B cells were treated with 40µM Roscovitine (RVT) for 24 hours. BrdU incorporation was analyzed as in (A). Cell lysates were immunoblotted for indicated proteins.