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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Gastroenterology. 2010 Jan 25;138(5):1920–1930. doi: 10.1053/j.gastro.2010.01.007

Figure 3. G1/S phase arrest by transcriptional repression of RB/E2F target genes is independent of RB status after PD-0332991 treatment.

Figure 3

(A) Cells were treated with increasing concentrations of PD-0332991 for 24 hours. Reverse transcription-PCR was done targeting the indicated genes. (B,C) Cells were treated as in (A) and cell lysates were analyzed by immunoblot for the indicated proteins. (D) Cells were transfected with luciferase reporter constructs pCycA-Luc, pCycA-CCREmut-Luc and CMV-ß-galactosidase expression plasmid. After transfection, cells were treated as in (A) and luciferase activity was measured. Average relative luciferase activities from at least three independent experiments; bars, SD.