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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Gastroenterology. 2010 Jan 25;138(5):1920–1930. doi: 10.1053/j.gastro.2010.01.007

Figure 5. E1A or E2F overexpression reverses cell cycle arrest in RB cells exposed to PD-0332991.

Figure 5

(A) Cells were co-transfected with H2B-GFP expression plasmid and indicated E1A expression plasmid, and treated with 1µM PD-0332991 for 24h. The percentage of BrdU-positive cells was determined from three independent experiments; bars, SD. (B) Cells were infected with adenoviruses harboring either GFP (vector) or E2F2. Cells were treated with 1µM PD-0332991 for 24 hours. Cells were pulsed with BrdU one hour before harvest. Cell cycle was analyzed by bivariate flow cytometry. (C) Infected cells treated 1µM PD-0332991 were harvested and analyzed by immunoblot for indicated proteins.