. Author manuscript; available in PMC: 2011 Jul 1.

Published in final edited form as: Protein Expr Purif. 2010 Jan 11;72(1):32–41. doi: 10.1016/j.pep.2010.01.008

Table 2B.

Increase in the Fluorescent Signal Upon Iron Removal^a

Construct	% Increase (Iron ➔ Apo)^b
Fe₂hTF	368^c
Fe_NhTF	74^c
N-lobe	266
Fe_ChTF	71^c
Fe_CTEV hTF	100
C-lobe	103

Iron-containing samples were assayed in 100 mM Hepes, pH 7.4 and apo-samples were generated by incubation in 100 mM Mes buffer, pH 5.6 containing 300 mM KCl and 4 mM EDTA.

Percent increase is calculated as 100 X [(F_apo- F_iron)/F_iron], in which F is the fluorescence intensity (in counts per second) at the λ_max.

From [15].