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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Protein Expr Purif. 2010 Jan 11;72(1):32–41. doi: 10.1016/j.pep.2010.01.008

Table 4.

Rate Constants for the process of iron releasea

Construct Iron release
k1 (min−1)		 Conformational
Event
k2 (min−1)		 Conformational
Event
k3 (min−1)
FeChTFb
(n= 6)		 0.79 ± 0.1 1.9 ± 0.6 -
FeCTEV hTF
(n= 4)		 0.81 ± 0.1 2.8 ± 0.5 -
C-lobeb
(n= 4)		 0.87 ± 0.1 1.8 ± 0.6 -
N-lobeb
(n= 9)		 53.7 ± 4.9 7.2 ± 1.6 1.2 ± 0.9
a

Iron release was monitored by fluorescence at pH 5.6 (100 mM Mes buffer containing 300 mM KCl and 4 mM EDTA) as described in Materials and Methods. Averages and 95% confidence intervals are presented for kinetic runs performed on different days (n= 4-9 as indicated). Each day, rapid mixings of sample (3-6) were made and kinetic traces averaged.

b

From [11]