Table 2.
Region (aa) | Length (bp) | PCR cycles | No. of clones |
Mutation |
P |
Mutation |
||
Expected sporadic | NSY | SYN | NSY/ SYN | |||||
1060-1069 | 30 | 70 | 90 | 0.25 | 1 | > 0.050 | 0 | 1/0 |
1070-1079 | 30 | 70 | 90 | 0.25 | 5 | < 0.001 | 3 | 5/3 |
1080-1089 | 30 | 70 | 90 | 0.25 | 4 | < 0.001 | 0 | 4/0 |
1090-1099 | 30 | 70 | 90 | 0.25 | 3 | < 0.001 | 5 | 3/5 |
1100-1109 | 30 | 70 | 90 | 0.25 | 5 | < 0.001 | 0 | 5/0 |
1110-1119 | 30 | 70 | 90 | 0.25 | 3 | < 0.001 | 1 | 3/1 |
1120-1129 | 30 | 70 | 90 | 0.25 | 1 | > 0.050 | 1 | 1/1 |
1130-1139 | 30 | 70 | 90 | 0.25 | 4 | < 0.001 | 0 | 4/0 |
1140-1149 | 30 | 70 | 90 | 0.25 | 0 | NS | 0 | 0 |
1150-1159 | 30 | 70 | 90 | 0.25 | 4 | < 0.001 | 0 | 4/0 |
1160-1169 | 30 | 70 | 90 | 0.25 | 0 | NS | 0 | 0 |
1170-1179 | 30 | 70 | 90 | 0.25 | 3 | < 0.001 | 2 | 3/2 |
1180-1189 | 30 | 70 | 90 | 0.25 | 6 | < 0.001 | 0 | 6/0 |
1190-1198 | 27 | 70 | 90 | 0.23 | 0 | NS | 0 | 0 |
1073-10811 | 27 | 70 | 90 | 0.23 | 4 | < 0.001 | 2 | 4/2 |
1131-11391 | 27 | 70 | 90 | 0.23 | 4 | < 0.001 | 0 | 4/0 |
1169-11771 | 27 | 70 | 90 | 0.23 | 3 | < 0.001 | 2 | 3/2 |
Epitope region. Expected sporadic mutations = ER × L × Nc × N × P/Ns, where ER = error rate of polymerases (2.02 × 10-6), L: Nucleotide length of compared region; Nc: Number of PCR cycles; N: Number of clones sequenced; P: Proportion of sporadic mutations expected to produce amino acid substitutions (2/3), Ns: Number of sample (1) according to Smith et al[37] (1997). PCR: Polymerase chain reaction; NS: No significant.