Table 2.

Codon usage in putative highly expressed and lowly expressed genes of C. glutamicum.

AA	Codon	High		Low		AA	Codon	High		Low
		N	RSCU	N	RSCU			N	RSCU	N	RSCU
Phe	UUU	149	0.17	1058	1.37	Ser	UCU	371	0.77	539	1.22
	UUC*	1608	1.83	481	0.63		UCC*	1972	4.11	284	0.64
Leu	UUA	19	0.03	486	0.78		UCA	175	0.36	360	0.82
	UUG	239	0.33	1284	2.06		UCG	59	0.12	676	1.53
	CUU	656	0.91	707	1.13	Pro	CCU	527	0.92	492	1.11
	CUC*	1583	2.21	341	0.55		CCC	185	0.32	205	0.46
	CUA	140	0.2	274	0.44		CCA*	1454	2.55	371	0.84
	CUG*	1666	2.32	651	1.04		CCG	119	0.21	705	1.59
Ile	AUU	412	0.43	1207	1.71	Thr	ACU	304	0.38	605	1.2
	AUC*	2477	2.57	654	0.93		ACC*	2758	3.45	427	0.85
	AUA	7	0.01	257	0.36		ACA	74	0.09	378	0.75
Met	AUG	1143	1	863	1		ACG	61	0.08	601	1.2
Val	GUU*	1495	1.45	883	1.11	Ala	GCU*	1707	1.18	957	1.08
	GUC*	1631	1.59	452	0.57		GCC*	1138	0.79	512	0.58
	GUA	336	0.33	396	0.5		GCA*	2529	1.75	789	0.89
	GUG	650	0.63	1443	1.82		GCG	411	0.28	1300	1.46
Tyr	UAU	47	0.07	632	1.41	Cys	UGU	64	0.44	213	1.3
	UAC*	1311	1.93	262	0.59		UGC*	230	1.56	114	0.7
TER	UAA	100	2.19	39	0.85	TER	UGA	4	0.09	47	1.03
	UAG	33	0.72	51	1.12	Trp	UGG	577	1	661	1
His	CAU	47	0.09	560	1.35	Arg	CGU	744	1.62	557	1.45
	CAC*	981	1.91	271	0.65		CGC*	1887	4.11	362	0.94
Gln	CAA	284	0.34	539	0.79		CGA	89	0.19	317	0.83
	CAG*	1385	1.66	830	1.21		CGG	16	0.03	489	1.27
Asn	AAU	141	0.13	795	1.37	Ser	AGU	19	0.04	483	1.09
	AAC*	1955	1.87	369	0.63		AGC	284	0.59	305	0.69
Lys	AAA	298	0.26	664	0.91	Arg	AGA	5	0.01	236	0.62
	AAG*	2014	1.74	800	1.09		AGG	13	0.03	341	0.89
Asp	GAU	959	0.55	1537	1.53	Gly	GGU	1026	0.93	1069	1.46
	GAC*	2559	1.45	468	0.47		GGC*	2830	2.55	562	0.77
Glu	GAA*	2117	1.04	1095	0.91		GGA	549	0.5	568	0.78
	GAG	1941	0.96	1313	1.09		GGG	26	0.02	724	0.99

N: the number of codons; AA: amino acid.

*Codon with significantly (P < .01) higher frequencies in highly expressed genes.

High: codons in highly expressed genes; Low: codons in lowly expressed genes.