Figure 3.

Time-lapse microscopy and single-cell analysis of daughter arrester cells growing in a microfluidic device shows specific arrest of daughter cells only in the presence of drug. Cells were seeded into a microfluidic device and grown in the absence of drug for 1 h before switching to either identical or drug-containing media. (A) When grown in the absence of drug, newly budded cells (outlined in red, green and blue) are fluorescent from G₁- to S-phase, and both mothers (outlined in yellow) and daughters progress through the cell cycle. When grown in the presence of drug (B), a mother cell (outlined in yellow) divides multiple times, but its daughter cells (outlined in red, green and blue) do not complete a cell division. (C) Average fluorescence intensity of single cells shown in (B) as a function of time. The peaks of fluorescence intensity have remarkably similar durations as well as magnitudes.