Table 1.

Oligonucleotide sequences used for the ligation-mediated assembly of a gene fragment of B. cereus

	Forward sequence		Reverse sequence
F1	GTATACTTCCAATCCAATGCA	R1	TCGCTTTCATTGCATTGGAT
F2	ATGAAAGCGAAGAAAAAAGATAAA	R2	ATATTCAGAAGTTTTATCTTTTTTCT
F3	ACTTCTGAATATCAGTACGTTG	R3	ACCATGCGTCAACGTACTG
F4	ACGCATGGTACTCTAATCAG	R4	CGGCCGTTCTGATTAGAGT
F5	AACGGCCGTAGCATCCC	R5	ACGTTTCCACGGGATGCTA
F6	GTGGAAACGTATCCCGTCC	R6	CACTTCGCTGGACGGGAT
F7	AGCGAAGTGAAACAGTTCCA	R7	CACCGGTCTGGAACTGTTT
F8	GACCGGTGAGGCGTTCA	R8	ACAGTTGAAGTTGAACGCCT
F9	ACTTCAACTGTTTCGCAACTGTTCAGCGTTTC

The similar starting sequences of F3 and F9 that led to side products during the assembly are in bold.