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Efficiency of several DNA modification methods for engineering M. genitalium genomes in yeast
| Method | Fraction of clones with the correct modificationa | Fraction of clones with a complete genomeb |
|---|---|---|
| Traditional sequence replacement | 0/97 | 0/22 |
| Tandem repeat pop-out | 0/38 | 1/9 |
| Tandem repeat endonuclease cleavage (TREC) | 28/28 | 10/10 |
| Cre/loxP recombinase | 28/30 | 4/4 |
aEstimated by diagnostic PCR.
bEstimated by multiplex PCR assay.
