. 2010 Mar 1;38(8):2594–2602. doi: 10.1093/nar/gkq123

Table 1.

PSA details and yield for assembly of 91-kb synthetic chromosome arm

PSA level	Number of products	Average product base pairs	Average (max.) sites blocked per sub-fragment	PSA vector	Fraction (number) of products selected		Total PSA yield (%)^c
PSA level	Number of products	Average product base pairs	Average (max.) sites blocked per sub-fragment	PSA vector	Culture^a	Clonal/plate^b	Total PSA yield (%)^c
0	64	1422	0.3 (2)	pCK	–	–	–
1	32	2844	0.8 (3)	pTS	0.69 (22)	0.22 (7)	91
2	16	5688	1.4 (4)	pCK	0.31 (5)	0.56 (9)	88
3	8	11 376	3.0 (6)	pTS	0.75 (6)	0	75
4	4	22 752	5.5 (11)	pCK	0.5 (2)	0.5 (2)	100
5	2	45 504	14.5 (19)	pTSBAC	0	1.0 (2)	100
6	1	91 010	–	pCKBAC	1.0 (1)	–	100

^aProducts selected in culture required a single digest screen.

^bFor products that were not selected directly in culture, four cfu were grown and screened via digestion.

^cPercent of products at each PSA level obtained from one round of culture/clonal screening.