Table 1.
Synthetic design tools
| Program name | Availability | Open source? | Manipulations offered | Batch processing? |
|---|---|---|---|---|
| GeneDesign (1) | Free webserver, source code downloadable scripts (PC/Mac/Linux/Unix) | Yes | Codon, restriction enzyme, reverse translation, large gene assembly, general sequence, assembly oligonucleotides | Yes |
| GenoCAD (7) | Free webserver | No | Construct assembly, semantic analysis | No |
| TmPrime (8) | Free webserver | No | Codon, large gene assembly, assembly oligonucleotides | No |
| OPTIMIZER (9) | Free webserver | No | Codon, restriction enzyme, reverse translation | No |
| Gene Designer (10) | Free executable (PC/Mac) | No | Codon, restriction enzyme, reverse translation, general sequence, sequencing oligonucleotides | No |
| Gene2Oligo (11) | Free webserver | No | Assembly oligonucleotides | No |
| DNAWorks (12) | Free webserver | No | Codon, restriction enzyme, reverse translation, assembly oligonucleotides | Yes |