Table 4.
Synthetic proteins constructed in this study
| ID | Compositiona | Size | Registry |
|---|---|---|---|
| Domain1 – [GS]5 – domain2 ⋈H6 | (kD) | IDb | |
| P01 | FKBP ∼ Luciferase 1 | 25.2 | J18933 |
| P02 | FKBP ∼ Luciferase 2 | 23.5 | J18934 |
| P03 | FKBP ∼ mCitrine | 42.1 | J18935 |
| P04 | FKBP ∼ mCherry | 41.9 | J18936 |
| P05 | FKBP ∼ mCerulean | 42.1 | J18937 |
| P06 | FKBP ∼ β-lactamase 1 | 34.6 | J18938 |
| P07 | FKBP ∼ β-lactamase 2 | 24.9 | J18939 |
| P08 | FRB ∼ Luciferase 1 | 24.6 | J18940 |
| P09 | FRB ∼ Luciferase 2 | 22.9 | J18941 |
| P10 | FRB ∼ mCherry | 41.2 | J18942 |
| P11 | ZipE34 ∼ Luciferase 1 | 18.4 | J18943 |
| P12 | ZipE34 ∼ mCerulean | 35.2 | J18944 |
| P13 | ZipR34 ∼ Luciferase 2 | 16.7 | J18945 |
| P14 | ZipE34 ∼ β-lactamase 1 | 27.8 | J18946 |
| P15 | LOV2 ∼ Luciferase 1 | 29.5 | J18947 |
| P16 | LOV2 ∼ Luciferase 2 | 27.8 | J18948 |
| P17 | Luciferase 1 ∼ LOV2 | 29.5 | J18949 |
| P18 | mCitrine ∼ LOV2 | 46.5 | J18950 |
| P19 | FRB ∼ mCitrine | 40.7 | J18951 |
| P20 | FRB ∼ mCerulean | 40.6 | J18952 |
| P21 | ZipE34 ∼ mCherry | 35.0 | J18953 |
| P22 | ZipR34 ∼ mCitrine | 35.5 | J18954 |
| P23 | LOV2 ∼ mCitrine | 46.5 | J18955 |
| P24 | LOV2 ∼ mCerulean | 46.3 | J18956 |
| P25 | mCerulean ∼ LOV2 | 46.3 | J18957 |
aComposition of fusion proteins—all proteins follow the same layout of domain 1, GS linker, domain 2, pre-Scission restriction site (
) and polyhistidine tag (
); see text for details.
bID in Registry of Standard Biological Parts (http://partsregistry.org); due to part assembly, all proteins start with the sequence MTG and end with TG. Proteins labeled in bold face were purified on large scale.