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. 2010 Mar 9;38(8):2645–2662. doi: 10.1093/nar/gkq152

Table 7.

FRET efficiencies for different combinations of interaction input and output devices

Protein pair	FRET method
	Donor quenching^a		Acceptor emmission^b
	Parallel^c	±rap.^d	Parallel^c	±rap.^d
FKBP∼mCerulean FRB∼mCitrine	0.23 ± 0.010	0.21 ± 0.007	0.20 ± 0.004	0.19 ± 0.015
FRB∼mCerulean FKBP∼mCitrine	0.19 ± 0.004	0.18 ± 0.004	0.18 ± 0.002	0.17 ± 0.008
FKBP∼mCitrine FRB∼mCherry	0.28 ± 0.006	0.25 ± 0.003	0.10 ± 0.010	0.10 ± 0.012
FRB∼mCitrine FKBP∼mCherry	0.29 ± 0.008	0.25 ± 0.004	0.09 ± 0.022	0.12 ± 0.022
Zip∼mCerulean Zip∼mCitrine	0.001 ± 3e-5		0.060 ± 0.006
Zip∼mCitrine Zip∼mCherry	0.017 ± 4e-4		0.021 ± 0.015

^aMeasuring quenching of donor fluorescence.

^bMeasuring sensitized acceptor emission.

^cComparing independent samples with and without donor (external control).

^dComparing fluorescence before and after adding rapamycin (internal control).