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. 2010 Mar 23;38(8):2607–2616. doi: 10.1093/nar/gkq165

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Example assembly graph. Example of an assembly graph for the goal part Pcon.RFP.GFP.term with real world (A) and abstract (B) representations. The primitive parts include a constitutive promoter (Pcon), red fluorescent protein coding sequence (RFP), green fluorescent protein coding sequence (GFP) and transcriptional terminator (term). These primitive parts are joined in two assembly steps to form composite parts Pcon.RFP and GFP.term during the first assembly stage. A second assembly stage, consisting of a single assembly step, completes construction of the goal part.