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. 2010 Apr 26;2010:671536. doi: 10.1155/2010/671536

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Copyright © 2010 Irina B. Alieva et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Microtubule plus-ends growth rates are different in centrosome region and on the periphery near the cell margin. Growing microtubule plus-ends were selectively marked in human EC. HPAEC were transfected with the plasmid expressing EB3-GFP. Persistent microtubule growth was confirmed by long EB3-GFP tracks. EB3-GFP movement was analyzed by time-lapse microscopy. Images were acquired every 1 second. Histogram of microtubule growth rate distribution were obtained by tracking EB3-GFP comets at microtubule plus-ends in HPAEC in the centrosome region (mean growth rate, 16.7 ± 0.3 μm/min (n = 82)) (a) and near the cell margin (mean growth rate, 12.9 ± 0.1 μm/min (n = 300)) (b).