Figure 2.

Dose–response relationship for UK-414,495 [((R)-2-({1-[(5-ethyl-1,3,4-thiadiazol-2-yl) carbamoyl]cyclopentyl}methyl) valeric acid)] in the anaesthetized rabbit. The amplitude of pelvic nerve-stimulated (PNS)-induced increase in vaginal blood flow (VBF) and the free plasma concentration of UK-414,495 were measured 45 min after infusion. The dose required to enhance VBF to 50% of the maximum achievable increase in flow was determined from this relationship. Arrows illustrate relationship between free concentrations of UK-414,495 and IC₅₀ potency against rabbit native neutral endopeptidase (NEP). All changes were monitored using laser Doppler technologies and data are expressed as mean ± SEM (n= 3–10), **P < 0.01 Student's t-test (paired vs. baseline control).