Table II. Peptide sequences identified from iterative MSn analyses of reduced CLR C1q B and CLR C1q C by direct infusion on LTQ-Orbitrap instrument.
% indicates loss of NH3 by the N-terminal glutamine. ND, not determined.
| Chain | Precursor ion for FTMS2m/za | Precursor ion for ITMS3 m/z | Identified sequences | Further ITMS4 |
|---|---|---|---|---|
| B | 1102.41 (10+) | 854.3670 (1+) | 1%QLSCTGPP*A | ND |
| 1104.02 (10+) | 967.4504 (1+) | 1%QLSCTGPP*AI | ND | |
| 1250.6014 (1+) | 1%QLSCTGP*PAIP*GI | ND | ||
| 1533.7533 (1+) | 1%QLSCTG(PP)*AIP*GIP*GI | ND | ||
| 535.3027 (2+) | 89YKATQKIAF97 | ND | ||
| C | 1256.71 (8+) | 1103.5312 (2+) | 71(PGP)*MGIP*GEPGEEGRYKQKF90 | ND |
| 736.0229 (3+) | 71P*GPMGIP*GEPGEEGRYKQKF90 | ND | ||
| 819.3975 (2+) | 77P*GEPGEEGRYKQKF90 | ND | ||
| 1258.71 (8+) | 1111.5269 (2+) | 71P*GPMGIP*GEP*GEEGRYKQKF90 | ND | |
| 741.3538 (3+) | 71P*GPMGIP*GEP*GEEGRYKQKF90 | ND | ||
| 827.3938 (2+) | 77P*GEP*GEEGRYKQKF90 | ND | ||
| 1314.23 (8+) | 1050.0091 (2+) | 77P*GEPGEEGRYKQKFQSVF94 | ND | |
| 889.7638 (3+) | 71P*GPMGIP*GEPGEEGRYKQKFQSVF94 | 399.17 | ||
| 1316.38 (8+) | 1058.0063 (2+) | 77P*GEP*GEEGRYKQKFQSVF94 | ND | |
| 895.0957 (3+) | 71P*GPMGIP*GEP*GEEGRYKQKFQSVF94 | 569.33 |
a The m/z value of the isotope of highest intensity in the pattern selected for fragmentation is provided.