Fig. 2.

TDP-S6 translocation and aggregation in HEK-293 cells and primary hippocampal neurons. A, HEK-293 cells were transfected with HA-TDP-43 or HA-TDP-S6 and stained with TDP-43 antibody for both recombinant and endogenous proteins (green), HA antibody for recombinant TDP-43 exclusively (red), and Hoechst stain for the nucleus (blue). The white bars represent a distance of 10 μm. B, neurons were transfected and stained with the neuron-specific marker enolase (green), HA antibody (red), and Hoechst stain (blue). Two different sections of the same neuron, corresponding to separate focal planes, are shown in the middle and bottom panels of TDP-S6 (Soma), indicating cytoplasm and nucleus, respectively. C, TDP-S6 was also aggregated in neuronal processes, shown under higher magnification.