Fig. 3.

Characterization of Sarkosyl-insoluble fraction by Western blotting and mass spectrometry. A, Western blot (WB) analysis of sarkosyl-soluble and -insoluble HA-TDP-43, native TDP-43, and HA-TDP-S6 using an anti-TDP-43 antibody. The asterisk (*) indicates phosphorylated forms. B, densitometry analysis of multiple TDP-43 forms. C, large scale profiling of Sarkosyl-soluble and -insoluble fractions by LC-MS/MS. Spectral counts of identified proteins were used as a semiquantitative index to derive probability values. snRNP, small nuclear ribonucleoprotein.