Fig. 5.

Quantitative proteomics of Sarkosyl-insoluble fraction by multiplex SILAC. A, diagram for biological replicates of SILAC analysis (experiments 1 and 2). The amino acid labeling order was swapped in the repeated analysis. B, silver-stained SDS gel of the isolated Sarkosyl-insoluble fractions. The gel lanes were excised as indicated. C, a representative fitting of a pairwise comparison in the first experiment, shown as a histogram fitted to a Gaussian curve. D, the experimental variations were similar in multiple comparisons, indicated by the values of S.D. L, light; M, medium; H, heavy; Ctl, control. GeLC-MS/MS is the method of one dimensional SDS gel and LC-MS/MS.