Fig. 6.

SILAC analysis of TDP insoluble proteome and characterization of SUMOylation. Shown are MS spectra of detected peptide ions for MOV10 (a negative control), TDP proteins, Ub, and SUMO-2/3 in one dimensional SDS gel and LC-MS/MS from the top gel band (>180 kDa) in experiments 1 (A) and 2 (B). C, Western blotting of SUMO-2/3 and HA in Sarkosyl-insoluble fractions from cells transfected with mock, TDP-43, and TDP-S6 plasmids. D, both Sarkosyl-soluble (10 μg) and -insoluble (1 μg) fractions were incubated with SENP2 followed by immunoblotting with antibodies against SUMO-2/3, TDP-43, and HA. The three bands indicated by arrows correspond to TDP-43 modified by one, two, or three SUMO-2/3 molecules, respectively. WB, Western blot.