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Localization of SUMO-2/3, polyubiquitin (Lys-48 and Lys-63), and TDP proteins in HEK-293 cells. Cells were transfected with plasmids expressing HA-TDP-43 or HA-TDP-S6 and then co-stained with HA antibodies (red), and other antibodies (green) against SUMO-2/3 (A) and Lys-48 (B) and Lys-63 (C) polyUb linkages. The nuclei were stained by Hoechst (blue). Nuclear SUMO-2/3 and TDP-43 co-localization is indicated by white arrowheads (A).
