Table I. Proteins secreted by two or more cell lines.
61 proteins were identified from two or more cell lines; proteins found reproducibly in three BON-1 CM analyses are shown in bold. Cellular location (Loc'n) was identified using Protein Center and extensive literature searches (C, cytoplasmic; E, extracellular and not constitutively membrane-bound; M, membrane-bound); in many cases more than one cellular location has been reported for proteins. Cell lines from which proteins were identified are indicated (B, BON-1; N, NCI-H727; S, SHP-77). Reference protein accession numbers are from the NCBI protein database (National Center for Biotechnology Information). Mass is the predicted mass of the precursor in daltons. Score is the Mowse score. Ion score is the highest Mowse score for individual peptides. MHC, major histocompatibility complex.
| Reference protein accession no. | Protein | Cell line | Loc'n | Mass | Score | Percent coverage | No. peptides | Ion score |
|---|---|---|---|---|---|---|---|---|
| NP_033562 | 14-3-3 protein ϵ | BNS | C | 29,043 | 171 | 34 | 12 | 67 |
| NP_006817 | 14-3-3 protein θ/τ | NS | C | 27,633 | 129 | 13 | 4 | 101 |
| NP_663723 | 14-3-3 protein ζ/δ | BS | C | 27,614 | 73 | 12 | 3 | 60 |
| NP_001605 | Actin γ | BNS | C | 41,662 | 151 | 5 | 6 | 106 |
| NP_006399 | AGR2 | BS | E | 19,848 | 46 | 14 | 4 | 37 |
| NP_000286 | α1-Antitrypsin | NS | E | 46,737 | 49 | 4 | 3 | 41 |
| NP_001624 | α1-Microglobulin | BS | E | 38,999 | 77 | 5 | 4 | 56 |
| NP_001123476 | α-Actinin 1 | BNS | C | 105,438 | 90 | 6 | 6 | 80 |
| NP_004915 | α-Actinin 4 | BNS | C | 104,723 | 90 | 6 | 9 | 80 |
| NP_000475 | Alzheimer disease amyloid β protein | BS | M, E | 86,944 | 141 | 11 | 12 | 75 |
| NP_001002858 | Annexin A2 | BS | C, E | 40,280 | 70 | 7 | 4 | 68 |
| NP_001145 | Annexin A5 | BNS | C, E | 35,806 | 55 | 8 | 5 | 56 |
| NP_004351 | Cadherin 1 | BS | M, E | 97,456 | 212 | 8 | 13 | 67 |
| NP_001009566 | Calsyntenin 1 | BNS | M, E | 109,662 | 135 | 6 | 9 | 89 |
| NP_006126 | CapZ α-1 | BN | C | 32,792 | 87 | 14 | 5 | 64 |
| NP_001864 | Carboxypeptidase E | BNS | E | 53,151 | 115 | 13 | 8 | 50 |
| NP_001279 | Chloride intracellular channel protein 1 | NS | M | 26,792 | 63 | 27 | 9 | 63 |
| NP_001266 | Chromogranin A | BNS | E | 50,688 | 95 | 3 | 4 | 64 |
| NP_001810 | Chromogranin B (secretogranin 1) | BNS | E | 78,276 | 72 | 5 | 5 | 72 |
| NP_003460 | Chromogranin C (secretogranin 2) | BNS | E | 70,810 | 97 | 7 | 5 | 81 |
| NP_005498 | Cofilin-1 | BS | C | 18,371 | 94 | 27 | 7 | 52 |
| NP_001814 | Creatine kinase B | BN | C | 42,513 | 120 | 8 | 2 | 90 |
| NP_000090 | Cystatin C | BS | E | 15,749 | 128 | 19 | 3 | 72 |
| NP_001952 | Elongation factor 2 (EF-2) | NS | C | 95,207 | 92 | 3 | 2 | 93 |
| NP_001419 | Enolase, α | BNS | C | 47,038 | 97 | 4 | 2 | 97 |
| NP_001967 | Enolase, β | NS | C | 46,801 | 167 | 10 | 6 | 100 |
| NP_001966 | Enolase, γ | BNS | C | 47,138 | 84 | 7 | 4 | 84 |
| NP_000025 | Fructose-bisphosphate aldolase | BNS | C | 39,289 | 49 | 9 | 2 | 49 |
| NP_000166 | Glucose-6-phosphate isomerase | NS | C, E | 63,016 | 103 | 7 | 6 | 66 |
| NP_036545 | Glutaminyl-peptide cyclotransferase | BS | C | 40,887 | 87 | 25 | 6 | 56 |
| NP_002037 | Glyceraldehyde-3-phosphate dehydrogenase | BNS | C | 35,922 | 49 | 6 | 4 | 49 |
| NP_004855 | Growth differentiation factor 15 | BS | E | 34,009 | 90 | 10 | 6 | 86 |
| NP_002131 | Heterogeneous nuclear ribonucleoprotein K transcript variant | 50,897 | 63 | 22 | 8 | 58 | ||
| NP_005336 | NS | C | 69,921 | 193 | 27 | 16 | 69 | |
| NP_002146 | Hsp 70 protein 1A/6/8 | 70,897 | ||||||
| NP_006588 | 70,767 | |||||||
| NP_005338 | Hsp 70 protein 5 | NS | C | 72,202 | 133 | 14 | 9 | 79 |
| NP_003290 | Hsp 90 β 1 (endoplasmin) | NS | C | 92,469 | 131 | 14 | 10 | 61 |
| NP_031381 | HSP90AB1 protein | BNS | C | 83,133 | 65 | 6 | 5 | 61 |
| NP_001017963 | HSP90AA1 protein | BNS | C | 98,030 | 421 | 25 | 20 | 99 |
| NP_002256 | Importin β, chain B | NS | C | 97,040 | 40 | 3 | 1 | 40 |
| NP_002207 | Inter-α-trypsin inhibitor heavy chain 2 | BS | E | 106,333 | 102 | 15 | 10 | 69 |
| NP_002264 | Keratin 8 | BS | C | 53,573 | 71 | 6 | 3 | 61 |
| NP_002291 | l-Lactate dehydrogenase, chain H | NS | C | 36,507 | 54 | 17 | 7 | 49 |
| NP_005557 | l-Lactate dehydrogenase, chain M | BS | C | 36,558 | 126 | 23 | 12 | 69 |
| NP_005558 | Mac-2-binding protein | BNS | E | 65,200 | 114 | 8 | 8 | 47 |
| NP_005908 | Malate dehydrogenase | BN | C | 36,295 | 155 | 29 | 11 | 64 |
| NP_002108 | MHC class I antigen; HLA-C | BS | M | 40,649 | 50 | 13 | 3 | 50 |
| NP_002406 | MIF | BS | E | 12,345 | 76 | 9 | 3 | 52 |
| NP_006175 | Nucleobindin 1 | NS | C | 53,748 | 77 | 2 | 2 | 77 |
| NP_002565 | Peroxiredoxin 1 | BS | C | 21,979 | 67 | 18 | 3 | 50 |
| NP_005800 | Peroxiredoxin-2 | BS | C | 21,761 | 45 | 5 | 2 | 45 |
| NP_005013 | Profilin | BS | C | 14,923 | 209 | 23 | 4 | 90 |
| NP_000468 | Serum albumin | BNS | E | 69,368 | 115 | 3 | 2 | 68 |
| NP_006746 | TALDO1 | NS | C | 37,409 | 56 | 8 | 6 | 46 |
| NP_003237 | Thrombospondin 1 | BS | E | 129,383 | 208 | 8 | 12 | 70 |
| NP_003245 | TIMP1 | BS | E | 23,189 | 50 | 5 | 2 | 50 |
| NP_000356 | Triose-phosphate isomerase | BS | C | 26,538 | 89 | 11 | 7 | 70 |
| NP_002760 | Trypsin (PRSS1) | BS | E | 26,558 | 43 | 12 | 4 | 43 |
| NP_003339 | Ubiquitin-protein ligase E2N | BS | C | 17,007 | 64 | 13 | 2 | 65 |
| NP_079493 | UL16-binding protein 2 | BS | M, E | 27,237 | 51 | 11 | 4 | 51 |
| NP_009057 | VCP | NS | C | 89,191 | 51 | 18 | 11 | 47 |
| NP_003369 | VGF, NGF-inducible | BNS | E | 67,275 | 152 | 22 | 18 | 97 |