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. 2010 May 15;137(10):1625–1633. doi: 10.1242/dev.046797

Fig. 6.

Fig. 6.

Expression of Pvf ligands is not lost in slit mutants. (A,B,F,G) In situ hybridisations on stage 12 wild-type (A,B) and slit mutant (F,G) Drosophila embryos show that Pvf3 is expressed along the midline on both the ventral (A,F) and dorsal (B,G) sides in both genotypes (arrows indicate the midline). (C,H) In stage 13/14 wild-type (C) and slit mutant (H) embryos, in situ hybridisation indicates that Pvf2 is expressed at the ventral midline (arrows). (D,I) Lateral views at stage 13/14 reveal Pvf2 expression to be restricted to the dorsal side of the VNC in wild-type embryos (D), but displaced from the dorsal aspect of the VNC in slit mutants (I). (E,J) High-magnification images of the ventral surface of embryos from D,I show tight clustering of the Pvf2 signal on the dorsal aspect of the VNC (arrow) in wild types (E), whereas some Pvf2-expressing cells are displaced ventrally (arrowheads) in slit mutants (J). Anterior is up. Scale bars: 20 μm.