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. Author manuscript; available in PMC: 2011 Apr 28.
Published in final edited form as: J Am Chem Soc. 2010 Apr 28;132(16):5779–5788. doi: 10.1021/ja910807e

Figure 7. A 2D PELF-WIM spectrum of aligned bicelles containing a mutant of Cytochrome b5 uniformly labeled with 15N isotope to compare the efficiencies of different PELF sequences.

Figure 7

Sample N-H dipolar coupling slices extracted from 2D PELF spectra, that were obtained using PELF sequences constituted with different magnetization transfer schemes, are included on the right side for a comparison of the efficiency of PELF sequences. In all of these cases, PELF with WIM or COMPOZER-CP offers a significant improvement in resolution particularly in the dipolar coupling dimension. The S/N (signal-to- noise) in the dipolar dimension of WIM, COMPOZER-CP and CP are 7.1, 7.5 and 8, respectively. The line-width taken at half height in the dipolar dimension for WIM, COMPOZER-CP and CP are approximately 150, 140, 400 Hz, respectively.