Methods for analyzing peptides and proteins on a chromatographic timescale by electron-transfer dissociation mass spectrometry

. Author manuscript; available in PMC: 2010 Apr 27.

Published in final edited form as: Nat Protoc. 2008;3(11):1709–1717. doi: 10.1038/nprot.2008.159

Method parameter	Value
Activation type	ETD
Reagent AGC target (radical anions)	2 × 10⁵ ion counts
Full AGC target (MS1 precursor cations)	1 × 10⁴ ion counts
MSn AGC target (MS2 isolated precursor cations)	2 × 10⁴ ion counts
Isolation window	3 m/z
MS1 scan range	300–2,000 m/z
Reaction time	100 ms*
Repeat count	2
Repeat duration	20 s
Exclusion duration	30 s