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. Author manuscript; available in PMC: 2010 Apr 27.
Published in final edited form as: Open Proteomics J. 2008 Jan 1;1:106–115. doi: 10.2174/1875039700801010106

Figure 1.

Figure 1

Protein displays in 2-DE gels of Y. pestis KIM6+ membrane fractions isolated with (A,C) or without (B,D) extraction using an alkaline Na2CO3 solution. Circa 100 μg protein was loaded onto an IPG strip (24 cm, pH 4–7), separated in the first dimension, followed by SDS-PAGE (8–16%T) in the second dimension. Gels were stained with Commassie Brilliant Blue G-250. pI and molecular mass ranges for the profiled proteins are denoted. Magnified gel segments of the denoted rectangles in A and B are presented in C and D. The arrows indicate spots identified as OmpA and Pla. Vertical and horizontal spot trains for OmpA and Pla were reproduced in numerous 2-DE experiments.