Table 2. Kinetic parameters of TEAS wild-type and the M4 enzyme determined using either (cis,trans)- or (trans,trans)-FPP.
(trans,trans)-FPP |
(cis,trans)-FPP |
|||||
---|---|---|---|---|---|---|
kcat (min−1) | KM (μM) | kcat/KM (μM−1 min−1) | kcat (min−1) | KM (μM) | kcat/KM (μM−1 min−1) | |
TEAS wt | 2.5 ± 0.7 | 8.4 ± 0.89 | 0.3 | 5.71 ± 0.08 | 14.03 ± 2.59 | 0.41 |
TEAS M4 | 9.43 ± 0.21 | 13.31 ± 1.04 | 0.71 | 4.62 ± 0.07 | 7.97 ± 1.26 | 0.58 |