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. 2010 May;12(3):292–299. doi: 10.2353/jmoldx.2010.090139

Figure 1.

Figure 1

The sensitivity of the TheraScreen K-RAS Mutation Kit in comparison with direct sequencing. Mixtures of DNA from KRAS mutant and wild-type FFPE tumors were used to compare the sensitivity of the two methods. A: For sample P08-274, the TheraScreen K-RAS Mutation Kit was able to detect the KRAS mutation G12V when the DNA from a mutant tumor represented 5% of the total DNA. The 1%ΔCt value is the cut-off level provided by the manufacturer to detect the presence of a G12V KRAS mutation and it is derived from cell lines and synthetic constructs. B: For the same sample, sequencing was unable to detect the KRAS mutation G12V when mutant DNA was present as <30% of the total DNA. Percentages indicate the proportion of DNA from a mutant tumor relative to DNA from a wild-type tumor.