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. 2010 May;12(3):368–376. doi: 10.2353/jmoldx.2010.090147

Figure 4.

Figure 4

Deletion of exons 6 to 8 detected by PCR and high-resolution melting. A: Normalized melting curves for exons 6 to 8 are shown for both wild-type (WT) controls and a 1:1 mixture of unknown patient and wild-type DNA (gray traces). However, when the unknown sample is tested without mixing, no specific melting curves are observed (black traces). B: Fluorescent image of the plate. Primers for exons 1 to 7 are in columns 1, 3, 5, 7, 9, and 11 and primers for exons 8 to 13 are in columns 2, 4, 6, 8, 10, and 12. Mixed DNA is present in columns 1 to 4, the unknown sample without mixing is present in columns 5 to 8 (exons 6 to 8 are boxed), and wild-type male DNA is present in columns 9 to 12.