Fig. 3.

Invertase expression in cells expressing Reg1 deletions. Invertase expression was assayed in cells grown in 2% glucose or after shifting to 0.05% glucose for three hours. The yeast strain (MSY990) containing a complete deletion of the REG1 gene was transformed with low copy number plasmids expressing REG1 mutants as shown. The mean invertase expression from three independent cultures is plotted with errors bars representing one standard error. Statistical significance was calculated in comparison to the wild type data in either high or low glucose. *, p < 0.05; **, p < 0.01; *** p < 0.001