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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cell Signal. 2010 Feb 17;22(7):1013–1021. doi: 10.1016/j.cellsig.2010.02.003

Fig. 5.

Fig. 5

Effect of Reg1 deletions on interaction with Snf1. (A) Reg1 forms a complex with Snf1. Protein extracts were prepared from cells (MSY987) expressing either untagged or HA-tagged Snf1 and untagged or V5-tagged Reg1 as indicated. Proteins were pulled down with HA-beads, eluted in SDS sample buffer, and probed by western blotting with V5 antibodies (top panel). (B) Protein extracts were prepared from cells expressing either wild type V5-tagged Reg1 (lanes 1 and 2) or the N1, C1 and C2 deletion mutants. (C) Protein extracts were prepared from cells expressing either wild type V5-tagged Reg1 (lanes 1 and 2), the C3 deletion mutant or the Reg1-IF point mutation.